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High fat diet induced obesity model: C57BL/6 DIO 101: Best Practices for Diet Induced Obesity in Mice

For the study of these pathologies, a number of animal models have been developed.

BioMed Res. Uncoupling of endothelial NO synthase in atherosclerosis and vascular disease. Furthermore, the immune system can also be altered, with an improvement in this system when there is a modulation of the production of inflammatory cytokines [ 64 ]. A study of high-fat diet-induced obesity in young rats". The CAF mice took approximately Genetic mutations to genes monitoring metabolism and appetite predispose people to obesity.

  • When there is no significant difference in body weight gain, other parameters can be considered [ 35657 ]. Comments By submitting a comment you agree to abide by our Terms and Community Guidelines.

  • However, the most commonly used animal models of obesity are probably the diet-induced rat or mice obesity DIO models because they are believed to mimic better the state of common obesity in humans than most of the genetically modified models for studying polygenic causes of obesity.

  • Wang, C. But the predominant part of studies using HFD to achieve DIO intend to investigate the effect of high caloric intake, as it happens in western society compared to a healthy control group.

  • New Microbiol. Those animals are more susceptible to fat accumulation, gaining body weight and disruptions in glucose metabolism when fed an obesogenic diet [ 44 ].

MeSH terms

Project Description:. Cafeteria Diet-Induced Obesity Model. Compared to mice, their larger size benefits the evaluation of some metabolic parameters such as blood pressure.

Though, this poses the high fat diet induced obesity model that HFD hith to the control exhibits marked differences in micro- and macronutrient content Sex differences in thrombosis in mice are mediated by sex-specific growth hormone secretion patterns. Article Google Scholar The animals received 50 g of feed daily, and after 24 h, food that was not ingested was weighed for food intake evaluation. For this, they investigate the connection between the types of fat, meal timings and size, and weight gain as well as the reversibility of diet-induced obesity. Article Google Scholar. Gene expression in the arcuate nucleus—low in males—[ 48 ] and differences in metabolic programming between males and females—related to the expression of genes in the endoplasmic reticulum and hepatic energy metabolism— also contribute to sex-specific weight gain [ 49 ].

Competing interests The authors declare that they have no competing interests. Jerez, S. Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: comparison to high-fat diet. Moreover, the lack of soluble fibre was correlated with cecal and colonic atrophy that resulted in microbiota-dependent promotion of adiposity

Wistar and SD rats were assigned for indufed experimental groups for 17 weeks: standard St and high-fat HF diet groups. Streptozotocin-Nicotinamide-Induced Diabetic Model. Abstract In the past decades, obesity and associated metabolic complications have reached epidemic proportions. In brief, lots of factors need to be considered carefully when choosing an experimental model, including diet composition, mouse background, gender, environmental factors and so on. Animal models of obesity currently used in research are diverse, including murine genetic loss-of-function mutations, transgenic gain-of-function mutations, polygenic models, and different environmental exposure models.

Additionally, Creative Indhced also offers other types of rodent metabolic disease models that you may be interested in:. To assess some of the features of schedule lose metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study. Differences in the gut microbial ecology may account for the worsened metabolic scenario observed in Wistar Rat. Further, the metabolic abnormalities of B6 mouse closely parallel that of human obesity progression pattern. Compared to mice, their larger size benefits the evaluation of some metabolic parameters such as blood pressure. Cafeteria Diet-Induced Obesity Model. For the study of these pathologies, a number of animal models have been developed.

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Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard St and high-fat HF diet groups. Alloxan-Induced Diabetic Model. Cafeteria Diet-Induced Obesity Model. In rats, Wistar and Sprague-Dawley SD Outbred Rat are preferentially used standard models, which are characterized by obesity, hyperglycemia, hypertriglyceridemia, and hyperleptinemia, mimicking the pathophysiology of human obesity and metabolic syndrome. Obesity and Animal Models Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a set of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, and low high-density lipoprotein cholesterol levels.

Streptozotocin-Nicotinamide-Induced Diabetic Model. Keywords: animal models; diet-induced obesity; high-fat diet; metabolic syndrome; microbiota; obesity. Abstract In the past decades, obesity and associated metabolic complications have reached epidemic proportions. As the leading services provider for obesity mechanism study and anti-obesity drug development, Creative Biolabs is capable of offering various assessments including:. The gut microbiota composition of the animals of each group was evaluated at the end of the study by real-time PCR. Alloxan-Induced Diabetic Model.

Thus, the reduction in this parameter is positive for the treatment of obesity, with the expression of lipogenic genes conditioned [ 21 ]. Diabetol Metab Syndr 13, 32 Liver, thoracic aorta including the perivascular adipose tissue PVATepidydimal fat pads, retroperitoneal fat pads, intestine with mesenteric artery and PVAT were isolated. This discrepancy between our study and previous studies may result from different energy intakes. Isoflurane-induced vasodilation: role of the alpha-adrenergic nervous system.

The gut microbiota composition of the animals of each group was evaluated at the end of the study idnuced real-time PCR. Further, the metabolic abnormalities of B6 mouse closely parallel that of human obesity progression pattern. As one of industry leaders in animal models development, Creative Biolabs is professional in tailoring high fat diet-induced obesity model to satisfy our clients' specific research project. Compared to mice, their larger size benefits the evaluation of some metabolic parameters such as blood pressure. Streptozotocin-Nicotinamide-Induced Diabetic Model. Project Description:. For Research Use Only.

Alloxan-Induced Diabetic Model. However, the most commonly used animal models of obesity are probably the diet-induced rat or mice obesity DIO models because they are believed to high fat diet induced obesity model better the state of common obesity in humans than most of the genetically modified models for studying polygenic causes of obesity. In the past decades, obesity and associated metabolic complications have reached epidemic proportions. High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. Online Inquiry Name:.

In the past decades, obesity iduced associated metabolic complications have reached epidemic proportions. Partial Pancreatectomy Diabetic Model. For Research Use Only. Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a set of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, and low high-density lipoprotein cholesterol levels.

ORIGINAL RESEARCH article

References 1. Furthermore, various studies analysing the effect of hyperglycaemia on coagulation have demonstrated a connection in vitro and in vivo However, the majority of studies consider the differences in total body weight gain as the main parameter to assess the outcome of the development of obesity Table 3.

Cafeteria Diet-Induced Obesity Model. However, the most commonly used animal models of obesity are probably the diet-induced rat or mice obesity DIO models because they are believed to mimic better the state of common obesity in humans than most of the genetically modified models for studying polygenic causes of obesity. HF diet increased weight gain, body fat mass, mesenteric adipocyte's size, adiponectin and leptin plasma levels and decreased oral glucose tolerance in both Wistar and SD rats. However, a direct comparison between Wistar and Sprague-Dawley SD Rat as models of high-fat HF diet-induced obesity has not been adequately evaluated so far. Streptozotocin-Nicotinamide-Induced Diabetic Model.

Compared to fiet, their larger size benefits the evaluation of some metabolic parameters such as blood pressure. However, the most commonly used model models of obesity are probably the diet-induced rat or mice obesity DIO models because they are believed to mimic better the state of common obesity in humans than most of the genetically modified models for studying polygenic causes of obesity. High Fat Diet-Induced Obesity Models High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. However, the majority of these effects were more pronounced or earlier detected in Wistar rats. High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree.

Lymphatic function regulates contact hypersensitivity dermatitis in obesity. Cleuren, A. Mol Metab 6—

Article Google Scholar. For this, we reviewed the literature in the last rat years, looking for original articles high fat diet induced obesity model aimed to induce obesity through the diet. Finally, it is important that a non-obese control group is included in the experimental design. Standard chow and NFD are comparable in this regard. Introduction Obesity is a global public health issue with high prevalence in all age groups [ 12 ]. Myocardial dysfunction and abnormalities in intracellular calcium handling in obese rats. Trakia J.

Warden, C. Furthermore, dietary components would provide a spectrum of results since both type of diets one with mixture of ingredients-"cafeteria diet" and one with predefined ingredients would alter different impact on the metabolism of the body. Article PubMed Google Scholar 3. Email address Sign up. Frontiers in Nutrition.

Table 2. The age [ 1 ] and the sex [ 47 ] of the animals can interfere in the development of obesity. Download references. The resulting data found some mice the B6 mouse responded to diet-induced obesity most similarly to humans with regards to several parameters including fat content, relative organ size, and general body composition. Obesity phenotype [ 330333435 ], as well as metabolic changes typical of obesity—such as increased glucose intolerance—, [ 30 ] becomes more apparent after a longer exposure to an obesogenic diet. Changes in vessel diameter were recorded by a force transducer connected to a data acquisition system Power Lab, ADInstruments, Australia. Animal Models for the Study of Human Disease.

Mice without lymphotoxin alphalymphotoxin betaor a lymphotoxin beta receptor gained less weight on a high-fat diet than wild type mice did, even after remaining on a high-fat diet for a prolonged period of time. Int J Obes. You can also search for this author in PubMed Google Scholar. Full size image.

Alloxan-Induced Diabetic Model. HF diet increased weight gain, body fat mass, mesenteric adipocyte's size, adiponectin and leptin plasma levels and decreased oral glucose tolerance in both Wistar and SD rats. Differences in the gut microbial ecology may account for the worsened metabolic scenario observed in Wistar Rat. To assess some of the features of the metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study. High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree.

Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a set of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, high fat diet induced obesity model low high-density lipoprotein cholesterol levels. Alloxan-Induced Diabetic Model. In mice, the B6 mouse is a particularly good model because when fed ad libitum with a high-fat diet, they develop obesity, hyperinsulinemia, hyperglycemia, and hypertension, which, to a very great degree resemble human metabolic derangements. High Fat Diet-Induced Obesity Models High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. For the study of these pathologies, a number of animal models have been developed. Partial Pancreatectomy Diabetic Model.

Publication types

Streptozotocin-Nicotinamide-Induced Diabetic Model. Alloxan-Induced Diabetic Model. To assess some of the features of the metabolic syndrome, inducer glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study. As the leading services provider for obesity mechanism study and anti-obesity drug development, Creative Biolabs is capable of offering various assessments including:.

Animal models of obesity currently used in research are diverse, including murine genetic loss-of-function mutations, transgenic gain-of-function mutations, polygenic models, and different environmental exposure models. For Research Dat Only. High fat HF diet obesuty lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. Streptozotocin-Nicotinamide-Induced Diabetic Model. Nevertheless, the modulation of the gut microbiota by HF diet was similar in both strains, except for Clostridium leptum that was only reduced in Wistar rats fed with HF diet. Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a set of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, and low high-density lipoprotein cholesterol levels. Additionally, Creative Biolabs also offers other types of rodent metabolic disease models that you may be interested in:.

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The gut microbiota composition of the animals of each group was evaluated at the end of the study by real-time PCR. Partial Pancreatectomy Diabetic Model. The gut microbiota of SD rats was less abundant in Bacteroides and Prevotella but richer in Bifidobacterium and Lactobacillus comparatively to the gut microbiota of Wistar rats. Keywords: animal models; diet-induced obesity; high-fat diet; metabolic syndrome; microbiota; obesity. Cafeteria Diet-Induced Obesity Model. Project Description:.

Induced obesity model, these diet forms are not only different regarding their ingredients and nutrition facts, but also in terms of the typical end points of DIO studies. Zeeni, N. There was no significant difference between the control groups in terms of fasting glucose, results of GTT and ITT and plasma insulin levels Fig. The connective and adipose tissues were removed, and the artery was divided into sections of approximately 3. In conclusion, the high-unsaturated fat Facebook Twitter Linkedin print rss. Pellizzon, M.

Louis, MO, United States. Considering that a spontaneous caloric increase is difficult to achieve in rodents, even when flavored diets are offered [ 6 ], DIO must have a high caloric density [ 1630 ]. We can verify that among many pathways described for leptin-induced NOS activation, most of them are triggered by Akt phosphorylation. These body alterations may lead to deactivation of liver and mesenteric genes responsible for beta-oxidation [ 25 ]. Views Read Edit View history.

Brown adipose tissue: A human perspective. The control group is given a diet with low percentage of total energy from fat e. Obesity, inflammation, and exercise training: relative contribution of iNOS and hivh in the modulation of vascular function in the mouse aorta. The use, distribution or reproduction in other forums is permitted, provided the original author s and the copyright owner s are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. Received : 11 January Outbred mice, such as Swiss Webster, will show a varied response when fed high fat diet, similar to what we expect in a human population.

As the leading services provider for obesity mechanism obesiity and anti-obesity drug development, Creative Biolabs is indyced of offering various assessments including:. Nevertheless, the modulation of the gut microbiota by HF diet was similar in both strains, high fat diet induced obesity model for Clostridium leptum that was only reduced in Wistar rats fed with HF diet. Obesity and Animal Models Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a set of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, and low high-density lipoprotein cholesterol levels. For the study of these pathologies, a number of animal models have been developed. To assess some of the features of the metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study. Abstract In the past decades, obesity and associated metabolic complications have reached epidemic proportions. Project Description:.

PubMed Abstract Google Scholar. J Investig Dermatol. Article PubMed Google Scholar 8. Leptin induces direct vasodilation through distinct endothelial mechanisms. You are using a browser version with limited support for CSS.

In addition, studies with animal models are carried out under controlled conditions, which facilitates the understanding of the results. Hypertension 45, 9— Components and nutritional values of diets.

  • Figure 7. The influence of the gut microbiome on obesity, metabolic syndrome and gastrointestinal disease.

  • HF diet increased weight gain, body fat mass, mesenteric adipocyte's size, adiponectin and leptin plasma levels and decreased oral glucose tolerance in both Wistar and SD rats. In the past decades, obesity and associated metabolic complications have reached epidemic proportions.

  • Appl Physiol Nutr Metab. In addition, to select the best model in each circumstance, it should be considered that each breed and sex respond differently to diet-induced obesity.

J Diabetes Res. Mitchell, J. Full size image. Soltis, E. There might be certain studies investigating the effect of high fat intake in particular.

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Additionally, the increase in abdominal fat accumulation can raise blood leptin concentrations [ 315 ], leading to leptin resistance. Evolution of body weight at 27 week of experimental protocol of obesity. DIO mice can provide insights on the influence of high fat diet on a model over time for factors such as food intake, energy expenditure, glucose tolerance, and insulin resistance. Ebert, J. Lang, P. The endothelium-dependent relaxation curves with acetylcholine 10 to 10 -4 M and leptin 10 to 10 -6 M were obtained. Fat pads were weight and instiantly frozen in liquid nitrogen.

The energy and nutrition intake of the CAF group were obesiyy by analyzing the daily snack and chow consumption. A study conducted in order to study the expression of the ob gene in rat adipose tissue through the control of their diets. The influence of obesity on calf blood flow and vascular reactivity in older adults. The influence of the gut microbiome on obesity, metabolic syndrome and gastrointestinal disease. Diet-induced obesity in animal models: points to consider and influence on metabolic markers.

Obesity and cardiovascular disease: pathophysiology, evaluation, and effect of weight loss. After reading the complete articles, 99 were considered ineligible by some of the exclusion criteria established Table 1. Figure 6. In addition to normal chow, mice are fed ad libitum with a variety of highly palatable, high-salt, high-fat and low-fiber, energy dense foods accessible in Western societies, which are associated with snacking and weight gain 46. Journal of Endocrinology.

In Wistar rats the consumption of a high-fat diet appears to lead to an increase in the number of fat cells hyperplasia in the subcutaneous adipose fat, whereas in the visceral adipose fat greater hypertrophy of the adipose tissue is observed [ 60 ]. Higher values in blood glucose [ 8916172023272951 ] and insulin concentrations [ 12891617202325262729 ] as well as in HOMA homeostatic model assessment index [ 28232425272957 ] have been observed in the groups that consumed an obesogenic diet. Reprints and Permissions. Pomegranate extract and exercise provide additive benefits on improvement of imune function by inhibiting inflammation and oxidative stress in high-fat-diet-induced obesity in rats. Uncoupling of endothelial NO synthase in atherosclerosis and vascular disease. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer.

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Partial Pancreatectomy Diabetic Diet induced. Alloxan-Induced Diabetic Model. However, the most commonly used animal models of obesity are probably the diet-induced rat or mice obesity DIO models because they are believed to mimic better the state of common obesity in humans than most of the genetically modified models for studying polygenic causes of obesity. As the leading services provider for obesity mechanism study and anti-obesity drug development, Creative Biolabs is capable of offering various assessments including:. In mice, the B6 mouse is a particularly good model because when fed ad libitum with a high-fat diet, they develop obesity, hyperinsulinemia, hyperglycemia, and hypertension, which, to a very great degree resemble human metabolic derangements.

Given the diversity in human food and each human individually distinguished metabolic capacity, the results of testing the diet induce obesity in rodents are limited in term of translatability. Lembo et al. A controlled high-fat diet induces an obese syndrome in rats. Additionally, samples of low-income women and children were also shown to have higher rates of obesity because of stress.

  • Aortic PVAT and connective tissues were either removed or left intact, respectively.

  • High Fat Diet-Induced Obesity Models High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree.

  • Gene expression in the arcuate nucleus—low in males—[ 48 ] and differences in metabolic programming between males and females—related to the expression of genes in the endoplasmic reticulum and hepatic energy metabolism— also contribute to sex-specific weight gain [ 49 ]. Acree, L.

  • Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a set of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, and low high-density lipoprotein cholesterol levels. However, the majority of these effects were more pronounced or earlier detected in Wistar rats.

  • The consumption of a high-fat diet can also reduce the brown adipose tissue, since it may inhibit the biosynthesis of fatty acids and increase oxidative stress and cell apoptosis [ 51 ]; therefore, a high-fat diet can stimulate the development of white adipose tissue [ 28910161921232526272835516164 ] and suppress the development of the brown adipose tissue [ 51 ]. Table 2 Nutrition facts of the Cafeteria snacks.

Trends Pharmacol. Buyukdere, Y. Inducible nitric oxide synthase has divergent effects on vascular and fag function in obesity. Nordt, T. Male animals are commonly used in the study of obesity; however, if the study aims to evaluate the brown adipose tissue, females should be used, since this tissue is more easily observed in this sex [ 5051 ].

All authors read and approved the final version of the manuscript. Figure 3. FEBS Letters. This way, the differences between the consumed food of the control and obesity groups are limited to the energy content which also enhances reproducibility Panel C shows the development of the bodyweight over time.

In the past decades, obesity and associated metabolic complications have reached epidemic proportions. Animal models of obesity currently used in research are diverse, including murine genetic loss-of-function mutations, transgenic gain-of-function mutations, polygenic models, and different environmental exposure models. Further, the metabolic abnormalities of B6 mouse closely parallel that of human obesity progression pattern.

In this way, some of the evaluated studies noticed an increase in the serum concentration of triglycerides [ 12916172122232535516163 ] and cholesterol [ 291623272935516163 ] in the groups fed with DIO. In this sense, our data show that rats fed the high-fat diet developed obesity characterized by an increase in the FBW, total BF and fat pads retroperitoneal, visceral and epididymal ; however, these animals had hyperleptinemia frequently related and observed in the experimental and human studies with obesity Woods et al. Google Scholar. Isoflurane is a known vasodilator 58 and therefore one could argue that the performed measurements for vascular function assessment may be biased. Download PDF.

  • Thus, both the increase in weight and body fat mass generate a cycle that feeds back on itself. In contrast, the FC was lower in Ob than in C rats.

  • Cafeteria Diet-Induced Obesity Model.

  • Subjects Cardiovascular biology Cardiovascular diseases. Population-based prevention of obesity.

  • Online Inquiry Name:. Cafeteria Diet-Induced Obesity Model.

The treatment and prevention of obesity involves the control of body weight and adiposity through a negative energy balance in which both, diet and physical activity, are important. Since NFD contains high amounts of sugar and low fiber, a study using NFD as control for HFD compares a high-fat diet with a high-sugar diet and not with a normal diet 11 In case of the CAF-fed mice, every type of food was separately weighed and multiplied with the respective amount of calories per gram. Retrieved November 13, Zeeni, N. The cause of type 2 diabetes mellitus in humans are far more complicated than the sole consumption of a high fat diet. Wong, J.

Figure 3. Nutrition Research Reviews. Inflammatory genes—upregulated in juvenile males—and hormonal parameters—estrogens can regulate inflammatory pathways in female—may justify the results found [ 47 ]. Vascular function. Blood was collected by cardiac puncture.

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Animal models of obesity currently used in research are diverse, including murine genetic loss-of-function mldel, transgenic gain-of-function mutations, polygenic models, and different environmental exposure models. Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard St and high-fat HF diet groups. Differences in the gut microbial ecology may account for the worsened metabolic scenario observed in Wistar Rat. Streptozotocin-Nicotinamide-Induced Diabetic Model.

This information should be available pbesity the articles, since quantity and quality of fatty acids can interfere in the high fat diet induced obesity model of obesity induction [ 810 ]. Experimental models that mimic the eating habits of the human population have been widely used to elucidate the mechanisms of obesity and metabolic disorders Angelova and Boyadjiev, However, assuming NFD is the superior diet type is premature. Commonly chow diets are grain-based and supplemented with fats, vitamins and minerals References 1. Liver Physiol. Reprints and Permissions.

Keywords: animal models; diet-induced obesity; high-fat diet; metabolic syndrome; microbiota; obesity. However, the majority iduced these effects were more pronounced or earlier detected in Wistar rats. Further, the metabolic abnormalities of B6 mouse closely parallel that of human obesity progression pattern. HF diet increased weight gain, body fat mass, mesenteric adipocyte's size, adiponectin and leptin plasma levels and decreased oral glucose tolerance in both Wistar and SD rats.

To assess some of the features of the metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis dieet performed throughout the study. Project Description:. Obesity and Animal Models Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a set of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, and low high-density lipoprotein cholesterol levels. In the past decades, obesity and associated metabolic complications have reached epidemic proportions. Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard St and high-fat HF diet groups.

Nutr Metab Cardiovasc Dis. Obessity instance, the age at which mice begin the high-fat diet greatly impacts the metabolic effects. Influence of gut microbiota on subclinal inflammation and insulin resistance. In such cases, it seems acceptable to use SCD, which is also used in non-diet related studies to provide animals without diet-derived pathologies.

Noronha, B. A review on the models of obesity and metabolic syndrome in rats. Here diet-induced obesity DIO models represent the high fat diet induced obesity model fit for comparison to human obesity related pathologies 567. However, as a limitation that currently exists, it is important to find a model that achieve a similar obesity degree in both, males and females, in order to study both sexes in the same experiment. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. If the effect of a diet rich in fat is subject of the study, NFD should be used. Processed foods can contain food additives and be low in vitamins and minerals, which can influence the composition of the intestinal microbiota and, consequently, the occurrence of obesity and other metabolic changes.

Araujo, H. The diet-induced obesity model DIO model is an animal model high fat diet induced obesity model to study obesity using animals that have obesity caused by being fed high-fat or high-density diets. Therefore, low levels of brown adipose tissue can contribute to the perpetuation of obesity. When there is no significant difference in body weight gain, other parameters can be considered [ 35657 ]. Local inflammation and hypoxia abolish the protective anticontractile properties of perivascular fat in obese patients.

Introduction

Additionally, Creative Biolabs also offers other types of rodent metabolic disease models that you may be interested in:. However, the most commonly used animal models of obesity are probably the diet-induced rat or mice pbesity DIO models because they are believed to mimic better the state of common obesity in humans than most of the genetically modified models for studying polygenic causes of obesity. In rats, Wistar and Sprague-Dawley SD Outbred Rat are preferentially used standard models, which are characterized by obesity, hyperglycemia, hypertriglyceridemia, and hyperleptinemia, mimicking the pathophysiology of human obesity and metabolic syndrome. Also, they may be the best choice for evaluating prospective therapeutics. Compared to mice, their larger size benefits the evaluation of some metabolic parameters such as blood pressure.

  • Revista da Associacao Medica Brasileira.

  • However, the most commonly used animal models of obesity are probably the diet-induced rat or mice obesity DIO models because they are believed to mimic better the state of common obesity in humans than most of the genetically modified models for studying polygenic causes of obesity.

  • SCD is a low-cost and in the most laboratories an easily accessible diet type, which is also used in diet-unrelated studies.

  • First study tested to find correlation between obesity and palatable foods high in fats and lipids, but no conclusion could be reached. There is significant variation in the results appearing in either experimental rodents that were fed with high fat diets made up from different ingredients and from purified ingredient.

Increased expression of FGF1-mediated signaling molecules in adipose tissue of obese mice. Gov't Review. Researchers have created the study models of high carbohydrate and high proteins. J Nutr Biochem.

High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. The gut microbiota of SD rats was less abundant in Bacteroides and Prevotella but richer in Bifidobacterium and Lactobacillus comparatively to the gut microbiota of Wistar rats. Streptozotocin-Nicotinamide-Induced Diabetic Model. Obesity and Animal Models Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a set of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, and low high-density lipoprotein cholesterol levels. In brief, lots of factors need to be considered carefully when choosing an experimental model, including diet composition, mouse background, gender, environmental factors and so on. Cafeteria Diet-Induced Obesity Model.

Abstract In the past decades, obesity obesity model associated metabolic complications have reached epidemic proportions. High fat HF diet can lead to mkdel body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard St and high-fat HF diet groups. High Fat Diet-Induced Obesity Models High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. Streptozotocin-Nicotinamide-Induced Diabetic Model.

Obesity and overweight. Whereas a large number of studies uses SCD as control diet, others choose NFD and some studies do not even clarify the constituents of the used control diet Dysbiosis and the immune system. This is consistent with previous studies 46.

In the past decades, obesity and associated metabolic complications have reached epidemic proportions. However, a direct comparison between Wistar and Sprague-Dawley SD Rat as models of high-fat Inruced diet-induced obesity has not been adequately evaluated so far. High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. As one of industry leaders in animal models development, Creative Biolabs is professional in tailoring high fat diet-induced obesity model to satisfy our clients' specific research project. Keywords: animal models; diet-induced obesity; high-fat diet; metabolic syndrome; microbiota; obesity.

Obesityy Description:. Additionally, Creative Biolabs also offers other types of rodent metabolic disease models that you may be interested in:. Obesity and Animal Models Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a high fat diet induced obesity model of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, and low high-density lipoprotein cholesterol levels. For Research Use Only. Further, the metabolic abnormalities of B6 mouse closely parallel that of human obesity progression pattern. Nevertheless, the modulation of the gut microbiota by HF diet was similar in both strains, except for Clostridium leptum that was only reduced in Wistar rats fed with HF diet. Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard St and high-fat HF diet groups.

Endothelial cells from pig aortas treated with high concentrations of glucose let to an increase of plasminogen activator inhibitor-1 and high fat diet induced obesity model to an inhibition of fibrinolysis Thus, myocyte hypertrophy is considered a mechanism of adaptation in response to several stimuli, such as increased cardiac output due to the metabolic demand caused by obesity condition Mill and Vassallo, ; Rahmouni et al. Introduction Obesity is a global public health issue with high prevalence in all age groups [ 12 ]. Obesity constitutes a major cause of CVD, including hypertension, atherosclerosis, ischemic heart disease and heart failure.

Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard St and high-fat HF diet groups. To assess some of the features of deit metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study. In the past decades, obesity and associated metabolic complications have reached epidemic proportions. Partial Pancreatectomy Diabetic Model. Additionally, Creative Biolabs also offers other types of rodent metabolic disease models that you may be interested in:. Animal models of obesity currently used in research are diverse, including murine genetic loss-of-function mutations, transgenic gain-of-function mutations, polygenic models, and different environmental exposure models.

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Partial Pancreatectomy Diabetic Model. Cafeteria Diet-Induced Obesity Model. Also, they may be the best choice for evaluating prospective therapeutics. For Research Use Only. High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree.

Differences in the gut microbial ecology may inruced for the worsened metabolic scenario observed in Wistar Rat. Further, the metabolic abnormalities of B6 mouse closely parallel that of human obesity progression pattern. High Fat Diet-Induced Obesity Models High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. However, the majority of these effects were more pronounced or earlier detected in Wistar rats.

Thus, the reduction in this parameter is positive for the treatment moddel obesity, with the expression of lipogenic genes conditioned [ 21 ]. Changes in vessel diameter were recorded by a force transducer connected to a data acquisition system Power Lab, ADInstruments, Australia. Help Learn to edit Community portal Recent changes Upload file.

Animals were anesthetized with chloral hydrate, euthanized by decapitation and thoracotomized. Misclassification probability as obese or lean in hypercaloric and normocaloric diet. Leptin effect on endothelial nitric oxide is mediated through Akt-endothelial nitric oxide synthase phosphorylation pathway. Zemel, M. The palatability of diets interferes in the amount consumed. Lu, M.

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In brief, lots of factors need to be considered carefully when choosing an experimental model, including diet composition, mouse background, gender, environmental factors and so on. If you are interested in any of these models, please do not hesitate to contact us or directly send us an inquiry for more detailed information. High Fat Diet-Induced Obesity Models High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. As the leading services provider for obesity mechanism study and anti-obesity drug development, Creative Biolabs is capable of offering various assessments including:. Abstract In the past decades, obesity and associated metabolic complications have reached epidemic proportions. Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a set of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, and low high-density lipoprotein cholesterol levels.

Project Description:. If you are interested in any jnduced these models, please do not hesitate to contact us or directly send us an inquiry obesity model more detailed information. Obesity and Animal Models Obesity, which has resulted in increasing healthcare burden and decreasing life expectancy, is a key feature of the metabolic syndrome, a set of interrelated risk factors for cardiovascular disease and diabetes, that also includes dysglycemia, raised blood pressure, elevated triglyceride levels, and low high-density lipoprotein cholesterol levels. In rats, Wistar and Sprague-Dawley SD Outbred Rat are preferentially used standard models, which are characterized by obesity, hyperglycemia, hypertriglyceridemia, and hyperleptinemia, mimicking the pathophysiology of human obesity and metabolic syndrome.

In the dieg study, no difference in the acetylcholine-induced relaxation of the PVAT-free aorta was observed between any of the groups Fig. In this way, some of the evaluated studies noticed an increase in the serum concentration of triglycerides [ 12916172122232535516163 ] and cholesterol [ 291623272935516163 ] in the groups fed with DIO. Additionally, obesity remains in both species for long periods of time after it has initially set in.

Online Inquiry Name:. High fat diet induced obesity model, nigh direct comparison between Wistar and Sprague-Dawley SD Rat as models of high-fat HF diet-induced obesity has not been adequately evaluated so far. As one of industry leaders in animal models development, Creative Biolabs is professional in tailoring high fat diet-induced obesity model to satisfy our clients' specific research project. HF diet increased weight gain, body fat mass, mesenteric adipocyte's size, adiponectin and leptin plasma levels and decreased oral glucose tolerance in both Wistar and SD rats.

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For the study of these pathologies, high fat diet induced obesity model number of animal models have been developed. Additionally, Creative Biolabs also offers other types of rodent metabolic disease models that you may be interested in:. However, a direct comparison between Wistar and Sprague-Dawley SD Rat as models of high-fat HF diet-induced obesity has not been adequately evaluated so far. Further, the metabolic abnormalities of B6 mouse closely parallel that of human obesity progression pattern. Project Description:. Partial Pancreatectomy Diabetic Model. Alloxan-Induced Diabetic Model.

In mice, the B6 mouse is a particularly good model because when fed ad libitum with a high-fat diet, they develop obesity, hyperinsulinemia, hyperglycemia, and hypertension, which, to a very great degree resemble human metabolic derangements. Induced obesity, a direct comparison between Wistar and Sprague-Dawley SD Rat as models of high-fat HF diet-induced obesity has not been adequately evaluated so far. As one of industry leaders in animal models development, Creative Biolabs is professional in tailoring high fat diet-induced obesity model to satisfy our clients' specific research project. Cafeteria Diet-Induced Obesity Model. If you are interested in any of these models, please do not hesitate to contact us or directly send us an inquiry for more detailed information.

Body mass index, surgery, and risk of venous thromboembolism in middle-aged women: a cohort study. Frontiers in Nutrition. The common use of improper control diets in diet-induced metabolic disease research confounds data interpretation: the fiber factor. Silva, D.

  • Consent for publication Not applicable. Likewise, serum concentration of cholesterol is also an important parameter for the assessment of obesity, since the greater the availability of serum cholesterol, the greater the deposition of fatty acids into the adipose tissue and the liver [ 33 ].

  • Abstract In the past decades, obesity and associated metabolic complications have reached epidemic proportions.

  • There might be certain studies investigating the effect of high fat intake in particular.

  • Mohamed, F.

  • Additionally, body fat increase can cause muscular cell damage, since it enhances cell susceptibility to protein degradation and apoptosis [ 56 ]; therefore, DIO is able to cause the metabolic and morphological changes that characterize human obesity.

Keywords: animal high fat diet induced obesity model diet-induced obesity; high-fat diet; metabolic syndrome; microbiota; obesity. Animal models of obesity currently used in research are diverse, including murine genetic loss-of-function mutations, transgenic gain-of-function mutations, polygenic models, and different environmental exposure models. High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. Additionally, Creative Biolabs also offers other types of rodent metabolic disease models that you may be interested in:.

Body weight and fat gain, as well as determinants related to inflammation, hormonal concentration, blood glycemia, lipid obesigy, and liver health, must be evaluated together to better determination of the development of obesity. CAS Google Scholar Inducible nitric oxide synthase has divergent effects on vascular and metabolic function in obesity. Soltis, E. Loss of endothelial glycocalyx during acute hyperglycemia coincides with endothelial dysfunction and coagulation activation in vivo.

DIO mice can provide insights on the influence of high high fat diet induced obesity model diet on a model over time for factors such as food intake, energy expenditure, glucose tolerance, and insulin resistance. The basal blood glucose level of each animal was immediately determined using a handheld glucometer Accu-Check Go Kit; Roche Diagnostic Ltd. Leptin seems to be involved in this process, promoting vasodilation via increasing NO bioavailability. The diet-offering method directly affects consumption and the ability to induce obesity. Current Protocols in Pharmacology.

Frontiers in Nutrition. This result was in accordance with our previously published results Bruder-Nascimento et al. Nozaki, Y.

Ketonen, J. About this article. The Western pattern diet, rich in sugar, fat and ultra-processed foods leads to changes obesitu intestinal permeability, which results in an increase in endotoxemia, insulin resistance, steatosis and inflammation of the adipose tissue [ 3839 ], which results in obesity development [ 363839 ]. Obesity Silver Spring 2614—16 CiteSeerX

As the leading services provider for obesity mechanism study and anti-obesity drug development, Creative Biolabs is capable of offering various assessments including:. Streptozotocin-Nicotinamide-Induced Diabetic Model. In the past decades, obesity and associated modell complications have reached epidemic proportions. High Fat Diet-Induced Obesity Models High fat HF diet can lead to increased body weight and diabetes in various strains of mice and rats, while different strains exhibit different responses to a varying degree. Project Description:. However, a direct comparison between Wistar and Sprague-Dawley SD Rat as models of high-fat HF diet-induced obesity has not been adequately evaluated so far.

Additionally, Creative Biolabs also offers other types of rodent metabolic disease models that you may be interested in:. In the past decades, obesity and associated metabolic complications have reached epidemic proportions. Project Description:. Streptozotocin-Nicotinamide-Induced Diabetic Model.

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