Obesity

Biology mr hoyle genetics and obesity: Publications by GMB Students

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Leptin levels were essentially unaltered by fetectomy. This agrees with prior reports that LEPR L transcripts typically occur in lesser abundance in peripheral tissues than those encoding short intracellular domain forms [ 12 ]. J Perinat Med 32 : — Sex-biased demographic events "sex-bias" involve unequal numbers of females and males. Leptin requirement for conception, implantation, and gestation in the mouse.

  • Am J Obstet Gynecol : — The vast majority of genome-wide association studies GWASs are performed in Europeans, and their transferability to other populations is dependent on many factors e.

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  • We perform phenome-wide analyses and directly measure the effect in homozygous carriers, commonly referred to as "human knockouts," across medical phenotypes for genes implicated as being protective against disease or associated with at least one phenotype in our study.

  • Decreases in placental LEP mRNA have been linked with decreased leptin concentrations in umbilical vein blood in cases of IUGR [ ], suggesting that leptin influences fetal growth in response to a fetal demand that is relative to placental supply [ ].

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Capacity for hormone production of cultured trophoblast cells obtained from placentae at term and in obesity pregnancy. Our data also help formulate biochemical models that may explain natural color variation in parrots. Finally, using a common genotype matrix, we show that eigenMT can be applied with even greater efficiency to studies across tissues or conditions. A positive umbilical venous-arterial difference of leptin level and its rapid decline after birth. J Clin Endocrinol Metab 84 : — The Chepang foragers harbor an elevated abundance of taxa associated with foragers around the world.

The first is the postpartum decline in leptin levels typically observed after the placenta is obeaity, although the decline in leptin is relatively prolonged for a hormone with such a short half-life. Increased plasma levels of adipokines in preeclampsia: relationship to placenta and adipose tissue gene expression. Nutrition 16 : —

  • Faulconnier YDelavaud CChilliard YInsulin and or dexamethasone effects on leptin production and metabolic activities of ovine adipose tissue explants. Population genetics theory predicts an increase of mutational load in populations undergoing serial founder effects during range expansions.

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  • We systematically characterized the transcriptome effects of protein-truncating variants, a class of variants expected to have profound effects on gene function, using data from the Genotype-Tissue Expression GTEx and Geuvadis projects. We sequenced genomes of the chocolate plant Theobroma cacao L.

  • Our findings contribute to a more complete reconstruction of pre-contact Caribbean population history and explore the role of Indigenous peoples in shaping the biocultural diversity of present-day Puerto Ricans and other Caribbean islanders. Increase in serum leptin and uterine leptin receptor messenger RNA levels during pregnancy in rats.

In this study, we used whole-genome data to assess the levels of heterozygosity in different lineages of the mangrove rivulus biology mr hoyle genetics and obesity infer the phylogenetic mg among those lineages. Expression intensity for leptin was greatest in early pregnancy, which mirrored the enhanced abundance of LEP transcripts at that time [ 29 ]. The relation of insulin, leptin and IGF-1 to birthweight in offspring of women with type 1 diabetes. Finally, using a common genotype matrix, we show that eigenMT can be applied with even greater efficiency to studies across tissues or conditions. The most significant peak was 6p

Progesterone and human placental lactogen inhibit leptin secretion on cultured trophoblast cells from human placentas biology mr hoyle genetics and obesity term. Joyle reconstruct the evolution of skin pigmentation, we collected phenotypes from over Khomani San and Nama individuals and high-throughput sequenced candidate pigmentation genes. View details for PubMedID Our requisition form analysis showed substantial heterogeneity in clinical laboratory ascertainment of REA, as well as marked incongruity among terms used to define REA categories. Accurate prediction of the functional effect of genetic variation is critical for clinical genome interpretation.

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We demonstrate that a subsequent unbiased next-generation sequencing approach correctly identified the driver mutation, and therefore may be more reliable for somatic variant detection. We assembled the Leontopithecus chrysopygus mitogenome, through analysis of M base pairs bp of short reads produced by next-generation sequencing NGS on the Illumina Platform, and investigated the presence of nuclear mitochondrial pseudogenes and heteroplasmic sites. Nat Med 3 : — Ontogeny and insulin regulation of fetal ovine white adipose tissue leptin expression.

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Placental leptin in normal, diabetic and fetal growth-retarded pregnancies. One might hypothesize, therefore, that, because the obesiy of Biology mr hoyle genetics and obesity mRNA transcripts in adipose tissue declined following fetectomy, increased estrogen levels in pregnancy would prompt commensurate increases in LEP transcripts in adipose tissue. Hum Reprod 16 : — Lappas MYee KPermezel MRice GERelease and regulation of leptin, resistin, and adiponectin from human placenta, fetal membranes, and maternal adipose tissue and skeletal muscle from normal and gestational diabetes mellitus-complicated pregnancies. Current population grouping methods to communicate these patterns are insufficient as they are inconsistent and fail to reflect the global distribution of genetic variability.

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To investigate positive selection on the dog lineage genetic in the domestication, we examined patterns of polymorphism in six canid genomes that were previously used to infer a demographic model of dog domestication. Like the human, the baboon possesses a true maternal-fetoplacental unit, which relies on androgen precursors from the fetal adrenal gland for placental estrogen synthesis [ 37 ]. Stretch-stimulated surfactant synthesis is coordinated by the paracrine actions of PTHrP and leptin. Longitudinal analysis of maternal plasma leptin concentrations during normal pregnancy and pre-eclampsia. Our results demonstrate that modeling population size change is necessary to estimate sex-bias parameters accurately.

  • DeepTag is trained on a newly curated dataset ofveterinary notes manually biiology by experts. Gender-based differences in the regulation of leptin synthesis are mediated by the steroid hormones [] and increases in serum leptin levels in early pregnancy may be owed to the stimulation of maternal adipose tissue by gestational steroids [].

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Whether shifts away from the foraging lifestyle that characterize much of humanity's past holye the gut microbiome, and to what degree, remains unclear. Analysis of cost dependence and running time on the data size suggests that, given near linear scalability, cloud computing can be a cheap and efficient platform for analyzing even larger sequencing studies in the future. Conversely, the gut microbiomes of the populations that have transitioned to farming are more similar to those of Americans, with agricultural dependence and several associated lifestyle and environmental factors correlating with the extent of microbiome divergence from the foraging population. Soluble leptin receptor and leptin levels in pregnant women before and after delivery. We employ a regularized estimator of the correlation matrix to ensure Meff is robust and yields adjusted p values that closely approximate p values from permutations.

Finally, on the basis of multiple linear regression analysis, we found that various correlates of recombination rate persist throughout the African great apes including repeats, diversity, and divergence. However, a principal challenge for genetic assays from genetlcs is the fragmented and chemically damaged state of DNA in formalin-fixed, paraffin-embedded Gfnetics samples. Biology mr hoyle genetics and obesity combined these data with population reference panels from Africa, Asia, Europe and the Americas to perform global ancestry analysis and infer the subcontinental origin of the European and Native American ancestry components of the admixed individuals. Circulating leptin levels during ovulation induction: relation to adiposity and ovarian morphology. I am also interested in clinical data science and development of new diagnostics. However, recent work demonstrated that, given a beacon with specific characteristics including relatively small sample size and an adversary who possesses an individual's whole genome sequencethe individual's membership in a beacon can be inferred through repeated queries for variants present in the individual's genome.

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We infer that the ancestors of western and eastern lowland gorillas diverged from a common ancestor approximately ka, and that the ancestors of the Cross River population diverged from the western lowland gorilla lineage approximately 68 ka. Indigenous peoples have occupied the island of Puerto Rico since at least B. A recent study cataloging variation in continental African populations suggests this type of African-specific genotyping array is both necessary and valuable for facilitating large-scale GWAS in populations of African ancestry. As expected, correlations between true and inferred risk are typically highest in the population from which summary statistics were derived. Using diffusion approximations, we modeled the demographic history of indigenous populations from Mexico with northern and southern ethnic groups splitting 7. In subcutaneous fat, leptin levels in fetectomized baboons were approximately one half that of controls, while in placenta levels were 3-fold higher in fetectomized animals than in those with intact pregnancies. Late Neolithic 3, BCE Moroccans, in contrast, share an Iberian component, supporting theories of trans-Gibraltar gene flow and indicating that Neolithization of North Africa involved both the movement of ideas and people.

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The soluble leptin receptor is crucial for leptin action: evidence from clinical and experimental data. Advance article alerts. While PCR-based genotyping methods abound in molecular testing for lung cancer therapy, these approaches may not provide the robust sensitivity to detect accurate genotypes in a variable cancer genomic background. Gynecol Endocrinol 18 : —

Digitization of human and veterinary health information will continue to be a reality, particularly in biology mr hoyle genetics and obesity form of kr narratives. Furthermore, gene-expression data from feathers of different bird species suggest that parrots acquired their colors through regulatory changes that drive high expression of MuPKS in feather epithelia. Here we analyse genome-wide variation in individuals from islands across Polynesia for signs of Native American admixture, analysing individuals from 17 island populations and 15 Pacific coast Native American groups. An augmented exome strategy provides a level of coverage not achievable with other platforms, thus addressing concerns regarding the lack of sensitivity in clinically important regions. Steroid-dependent up-regulation of adipose leptin secretion in vitro during pregnancy in mice.

Here, we characterize the effect of 18, protein-truncating variants across phenotypes from the UK Biobank and find 27 associations between medical phenotypes and protein-truncating variants in genes outside the major histocompatibility complex. Peptides 26 : — Ovariectomy was also reported to reduce serum leptin levels in humans [ ]. Some populations notably those in the Neotropics and the South Pacific are almost completely derived from European stock, whereas others are clearly admixed between indigenous and European dogs.

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Proponents have pointed to the existence of New World bioligy, such as the sweet potato and bottle gourd, in the Polynesian archaeological record, but nowhere else outside the pre-Columbian Americas, while critics have argued that these botanical dispersals need not have been human mediated7. Augmentation of leptin and hypoxia-inducible factor 1 alpha mRNAs in the pre-eclamptic placenta. Therefore, we developed a novel framework to select tag SNPs using the reference panel of 26 populations from Phase 3 of the Genomes Project. Despite recent genomic approaches to reconstruct the continental evolutionary history, regional characterization of ancient and modern genomes remains understudied. Fluctuation of serum leptin level in rats after ovariectomy and the influence of estrogen supplement. Cite Cite Michael C. Second, we conducted semi-structured interviews of 19 key-informants from 10 Mexican states remotely with digital recording and transcription.

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  • In collaboration with the Clinical Genomic Resource ClinGen -the flagship NIH program for clinical curation-we propose the first machine learning system, LitGen, that can retrieve papers for a particular variant and filter them by specific evidence types used by curators to assess for pathogenicity.

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Three of these changes are missense mutations in genes that produce male-specific minor histocompatibility H-Y antigens. Medulloblastoma is associated with rare hereditary cancer predisposition syndromes; however, genetics and medulloblastoma predisposition genes have not been defined and screening guidelines for genetic counselling and testing for paediatric patients are not available. Obstet Gynecol : — There was also striking disparity across REA populations in the amount of information available about clinically relevant variants in gnomAD. Related articles in Web of Science Google Scholar. Here we de novo assemble a reference genome for the naturally short-lived African turquoise killifish, providing a unique resource for comparative and experimental genomics.

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Hum Reprod 18 : — As much of the health data in the country becomes digitized, our visualization tools allow a digital dashboard of the hospital service utilization histories. Fertil Steril 70 : —

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  • Transcriptional regulation of the leptin gene promoter in rat GH3 pituitary and C6 glioma cells. Compared to recent sex-bias inference methods, our approach can detect sex-bias that changes on a single population branch without requiring data from an outgroup or knowledge of divergence events.

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Eur J Endocrinol : — A recent study cataloging variation in continental African populations suggests this hoylf of African-specific genotyping array is both necessary and valuable for facilitating large-scale GWAS in populations of African ancestry. We also analyze patterns of variation among western lowland gorillas to identify several genomic regions with strong signatures of recent selective sweeps. J Reprod Med 47 : — Clin Sci 95 : —

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Leptin functions via a specific receptor that is a member of the class I cytokine receptor superfamily and is manifested in alternatively spliced isoforms that are distinguished hoyld the relative lengths of their cytoplasmic regions. Reprod Nutr Dev 43 : — Here, we expand upon the work of the Great Ape Genome Project by analyzing medium to high coverage whole-genome sequences from 14 western lowland gorillas Gorilla gorilla gorilla2 eastern lowland gorillas G. In another group of pregnant baboons, estrogen production was inhibited at Day by fetectomy. Circulating leptin levels during ovulation induction: relation to adiposity and ovarian morphology.

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J Clin Endocrinol Metab 87 : — Despite this limitation, using existing software and a set of simple heuristics, we generated a final genotype matrix containing SNPs from 89 DNA samples from a single lane of Illumina HiSeq and kr it to create a saturated genetic linkage map and to identify a known QTL underlying apple skin color. We present egnetics linkage-disequilibrium LD -based recombination maps generated using whole-genome sequence data from 10 Nigerian chimpanzees, 13 bonobos, and 15 western gorillas, collected as part of the Great Ape Genome Project Prado-Martinez J, et al. REVEL was trained with recently discovered pathogenic and rare neutral missense variants, excluding those previously used to train its constituent tools. Microarray analysis of differentially expressed genes in placental tissue of pre-eclampsia: up-regulation of obesity-related genes. Whole exome sequencing is increasingly used for the clinical evaluation of genetic disease, yet the variation of coverage and sensitivity over medically relevant parts of the genome remains poorly understood. Previously, rare mutations in low-density lipoprotein LDL genes have been shown to contribute to MI risk in individual families, whereas common variants at more than 45 loci have been associated with MI risk in the population.

Am J Perinatol 22 : — Directional secretion and transport of leptin and expression of leptin receptor isoforms in human placental BeWo cells. Controlling for background demographic effects is important for accurately identifying loci that have recently undergone positive selection. Sign In or Create an Account.

Opportune IHC testing was found to be beneficial when it was available before or during the first rounds of chemotherapy. Similarly, it was reported that DHEA [ ], DHEA-sulfate, androstenedione, and nonaromatizable dihydrotestosterone [ 94 ] are potent inhibitors of leptin secretion by adipose tissue [ hoyle genetics and. Hyole Pulmonol 32 : 76 — Because of the wealth of research published over the last decade concerning its importance during pregnancy, we will focus primarily on the years following our last minireview of the subject [ 9 ]. Hum Reprod 14 : — The Norwegian explorer Thor Heyerdahl controversially suggested that prehistoric South Americanpopulations had an important role in the settlement of east Polynesia and particularly of Easter Island Rapa Nui 2. We show that fewer hotspots are shared among chimpanzee subspecies than within human populations, further narrowing the time scale of complete hotspot turnover.

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  • Gestational profile of leptin messenger ribonucleic acid mRNA content in the placenta and adipose tissue in the rat, and regulation of the mRNA levels of the leptin receptor subtypes in the hypothalamus during pregnancy and lactation.

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African Americans have a higher incidence of venous thromboembolism VTE than European descent individuals. Our study is the first to analyze within- and between-species genome-wide recombination rate biology mr hoyle genetics and obesity in several close relatives. Recently developed DNA library preparation methods for NGS have helped solve this problem, however, by combining the use of reduced representation libraries with DNA sample barcoding to generate genome-wide genotype data from a common set of genetic markers across a large number of samples. We systematically characterized the transcriptome effects of protein-truncating variants, a class of variants expected to have profound effects on gene function, using data from the Genotype-Tissue Expression GTEx and Geuvadis projects. Subsequent observations from monochorionic twin pregnancies revealed that fetal and cord leptin levels were at least 2-fold higher in normal-size fetuses than in their growth-restricted twins [ ], indicating a pivotal role in regulating growth [ ].

Skin pigmentation is under strong directional selection in northern European and Asian populations. Hormonal and body composition predictors of soluble leptin receptor, leptin, and free leptin index in adolescent biology mr hoyle genetics and obesity with anorexia nervosa and controls with relation to insulin sensitivity. Leptin concentrations rise along with estrogen and are correlated in early pregnancy with those of hCG. Sooranna SRWard SBajoria RDiscordant fetal leptin levels in monochorionic twins with chronic midtrimester twin-twin transfusion syndrome. In collaboration with the Clinical Genomic Resource ClinGen -the flagship NIH program for clinical curation-we propose the first machine learning system, LitGen, that can retrieve papers for a particular variant and filter them by specific evidence types used by curators to assess for pathogenicity.

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Leptin receptor expression in biology mr hoyle genetics and obesity lung increases yenetics late gestation in the baboon: a model for human pregnancy. Here we de novo assemble a reference genome for the naturally short-lived African turquoise killifish, providing a unique resource for comparative and experimental genomics. Clin Chem Lab Med 40 : — In light of the differential genetic architecture that is known to exist between populations, bias in representation can exacerbate existing disease and healthcare disparities. Although preeclampsia-associated hyperleptinemia has also been linked to enhanced solLEPR [ ], conflicting reports [] call for further investigation.

Cord leptin levels in diabetic pregnancies were strongly correlated with both conceptus growth and the degree of glycemic control [], and among the offspring of gestational diabetics, serum leptin levels were enhanced over population norms until at least 9 yr of age [ ]. Progression-free survival and overall survival were modelled for patients with a genetic predisposition to medulloblastoma. Here, we characterize the effect of 18, protein-truncating variants across phenotypes from the UK Biobank and find 27 associations between medical phenotypes and protein-truncating variants in genes outside the major histocompatibility complex. Similarly, levels in female fetuses [ 56 ] and neonates [ 78 ] are higher than in their male counterparts and levels in premenopausal women may be greater than those following menopause [as reviewed, 9—11]. Using ancestry tract length analysis we modeled post-colonial South American migration history as the youngest in Latin America during European colonization generations agowith an additional strong pulse of European migration occurring between 3 and 9 generations ago. Also in rats, ovariectomy diminished Lep gene expression in white adipose tissue and caused a decline in serum leptin levels [], while administration of E 2 reversed all the effects of ovariectomy.

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Am J Obstet Biology mr hoyle genetics and obesity : — Critical variants may be missed if they have a low frequency or are completely absent in European populations, especially as the field shifts its attention towards rare variants, which are more likely to be population-specific We conclude that there is a strong signal of purifying selection at conserved genomic positions within Africa, but that many predicted deleterious mutations have evolved as if they were neutral during the expansion out of Africa. The relationship of the levels of leptin, insulin-like growth factor-I and insulin in cord blood with birth size, ponderal index, and gender difference. At low-density lipoprotein receptor LDLRcarriers of rare non-synonymous mutations were at 4. In light of the differential genetic architecture that is known to exist between populations, bias in representation can exacerbate existing disease and healthcare disparities. Leptin receptors are down-regulated in uterine implantation sites compared to interimplantation sites.

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Indeed, there is some disagreement as to whether the mouse placenta produces any leptin at all [ 35 ]. Just as estrogen and corticosteroids are associated with the enhancement of leptin synthesis, androgens are linked to leptin inhibition [ ]. Obesity fast mediates the parathyroid hormone-related protein paracrine stimulation of fetal lung maturation. The country's average ancestry was 0. However, the timing and magnitude of these events resulted in markedly different patterns of admixture throughout Latin America. The KBTBD8 gene has been associated with idiopathic short stature and we found it to be highly differentiated in Triqui, a southern indigenous group from Oaxaca whose height is extremely low compared to other Native populations. Our findings highlight the importance of implementing and supporting programs to increase diversity in genome sequencing and clinical genomics, as well as measuring uncertainty around population-level datasets that are used in variant interpretation.

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Despite this, details of the timing and mode geneticcs selection at DARC remain poorly understood. We have applied our classifier to compare call sets generated with different calling methods, including both single-sample and multi-sample callers. Biol Reprod 67 : — Acta Obstet Gynecol Scand 83 : — GBStools is most effective at removing genotype errors in data sets over samples when coverage is 40X or higher, and the improvement is most pronounced in species with high genomic diversity. J Biol Chem : —

Replication was evaluated in Latinos, European Americans, and African Americans from independent nr. Our findings highlight the importance of implementing and supporting programs to increase diversity in genome sequencing and clinical genomics, as well as measuring uncertainty around population-level datasets that are used in variant interpretation. We hypothesized that genetic factors instead drive variation in timing. Life Sci 64 : —

Comprehensive genettics analysis, including analysis of exon co-association in the same molecule, requires deep, long-read sequencing. I am also interested in disruptive innovation for healthcare including modeling long-term risk shifts and novel payment models. A major rate limiting step in clinical interpretation is the manual curation of evidence in the genetic literature by highly trained biocurators. Expression of placental leptin and leptin receptor transcripts in early pregnancy and at term. In this capacity, the obligatory nature of leptin signaling in mammalian implantation [ 68 ] was illustrated by experiments in the mouse that demonstrated that endometrial leptin receptor expression was pregnancy dependent and that intrauterine injection of a leptin peptide antagonist or a leptin antibody impaired implantation. The genetic history of African rainforest hunter-gatherers and neighboring farmers is characterized by an ancient divergence more thanyears ago, together with recent population collapses and expansions, respectively [].

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Second, we conducted semi-structured ovesity of 19 key-informants from 10 Mexican states remotely with digital recording and transcription. Using strategies tailored for analysis of multi-ethnic and admixed populations, genetics and obesity describe a framework for analysing diverse populations, identify 27 novel loci and 38 secondary signals at known loci, as well as replicate 1, GWAS catalogue associations across these traits. Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa. Commonly used RV association tests have limited scope to leverage variant functions. Large-scale whole-genome sequencing studies have enabled the analysis of rare variants RVs associated with complex phenotypes. Hyperleptinemia of pregnancy associated with the appearance of a circulating form of the receptor.

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